Invitrogen™ LeukoLOCK™ Fractionation & Stabilization Kit with Manual
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1933-Each-of-1 | In Stock | ₹ 57,838.56 |
AM1933 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 57,838.56
GST (18%): ₹ 10,410.941
Total Price: ₹ 68,249.501
Concentration
1X
Isolation Technology
Vacuum Filter Column, Magnetic Bead
Final Product Type
Cells (Leukocytes)
For Use With (Application)
Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting
Prep Scale
> 1 mL Blood Samples (Large-scale)
Product Type
Kit
Starting Material Amount
Up to 10 ml whole blood
Yield
20 μg
Content And Storage
The RNAlater™ Solution, Rubber Septum Caps, White Slip Connectors, Transfer Spikes, LeukoLOCK™ Filters, and Elution Solution, should be stored at room temperature. 1X PBS may be stored at any temperature.
Sample Type
Blood
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Includes
RNAlaterSolution, Rubber Septum Caps, White Slip Connectors, Transfer Spikes, LeukoLOCK Filters, and Elution Solution, and 1X PBS
Product Line
Ambion™, LeukoLOCK™
Shipping Condition
Room Temperature
Target
Total RNA
Related Products
Description
- The Ambion™ LeukoLOCK™ Fractionation & Stabilization Kit is one of two kits that comprise the Total RNA Isolation System (patent pending), an innovative method for cellular fractionation of whole blood and total RNA stabilization and extraction from the leukocyte population
- The kit includes sufficient reagents for 20 reactions
- The LeukoLOCK™ System (Cat
- No
- AM1923) is optimized for use with human blood
- Blood is a storehouse of cellular information; however, the presence of globin mRNA in RNA prepared from whole blood can interfere with downstream expression profiling applications
- The LeukoLOCK™ System employs filter-based leukocyte-depletion technology to isolate leukocytes from whole blood and Ambion's RNA later ™ Solution to stabilize the cells on the filter
- By excluding red blood cells, the RNA that is purified from captured leukocytes is inherently depleted of globin mRNA, which improves sample utility for expression profiling and other applications (see Figure)
- LeukoLOCK™ Fractionation & Stabilization Kit A 9–10 mL sample of anticoagulated blood is passed through a LeukoLOCK™ filter, which captures the total leukocyte population while eliminating red blood cells (including reticulocytes), platelets, and plasma
- After rinsing with phosphate-buffered saline, the filter is flushed with RNA later ™ Solutionto stabilize the RNA in the captured leukocytes (see protocol schematic)
- The RNA can be isolated immediately, or stabilized cells can be maintained for several days at room temperature or for longer periods at –20°C or –80°C (see Figure)
- Accessory Products: LeukoLOCK™ Fractionation & Stabilization Kit is designed for use with the LeukoLOCK™ Total RNA Isolation Kit (Cat
- No
- AM1934)
- These kits are offered separately to accommodate blood sample collection at one location and sample processing at a different location, but may be purchased together as the LeukoLOCK™ Total RNA Isolation System (Cat
- No
- AM1923)
- Order Info Shipping Condition: Room temperature