Invitrogen™ RiboPure™ RNA Purification Kit, blood
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1928-Each-of-1 | In Stock | ₹ 57,325.20 |
AM1928 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 57,325.20
GST (18%): ₹ 10,318.536
Total Price: ₹ 67,643.736
Content And Storage
• 25 mL Elution Solution (4°C)• 40 mL Lysis Solution (4°C)• 25 mL Acid-Phenol:Chloroform (4°C)• 5 mL Sodium Acetate Solution (4°C)• 35 mL Wash Solution 1 (room temperature/4°C)• 70 mL Wash 2/3 Concentrate (room temperature/4°C)• 100 mL RNAlater™ Solution (room temperature)• 40 Filter Cartridges (room temperature)• 80 Collecion Tubes (room temperature)• 100 μL DNase I, 8 U/μL (-20°C, frost-free)• 850 μL 20X DNase Buffer (-20°C)• 1.2 mL DNase Inactivation Reagent (-20°C)• 1 mL Formaldehyde Load Dye (-20°C)This kit contains enough reagents for 40 RNA isolation from 300-500 μL whole blood.
Isolation Technology
Organic Extraction, Spin Column
Sample Type
Blood
High-throughput Compatibility
Not High-throughput Compatible (Manual)
No. of Reactions
40 Preps
Product Line
Ambion™, RiboPure™
Starting Material Amount
Up to 0.5 ml whole blood
Yield
4 μg
Format
Filter Cartridge, Tube
Purification Time
30 min.
Final Product Type
Total RNA
For Use With (Application)
Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Prep Scale
100 μL to 1 mL Blood Samples (Medium-scale)
Quantity
40 preps
Target
Total RNA
Related Products
Description
- The RiboPure™-Blood Kit is for the isolation of high-quality RNA directly from whole blood
- The kit combines two methods of RNA purification to efficiently remove blood components (protein, heme, genomic DNA, and RNases) that can compromise downstream procedures
- The kit includes sufficient reagents for 40 RNA purifications from whole blood samples ranging from 0.3–0.5 mL each
- Advantages of the RiboPure™-Blood Kit: • Purify total RNA directly from whole blood—without prior recovery of WBCs • Simple, fast procedure—from blood to RNA in less than 30 minutes • Includes RNA later ™ Solution—store blood samples 3 days or more at room temperature • Includes DNA- free ™ Reagents—to ready RNA for RT-PCR and array analysis Applications The RiboPure™-Blood Kit produces high yields of pure RNA directly from whole blood, eliminating the need to isolate WBCs prior to RNA purification
- RNA recovered using the RiboPure™-Blood Kit can be used in the most challenging applications, including real-time RT-PCR and array analysis
- Average yields of RNA are between 2–4 μg/0.5 mL of whole blood (variation is due to differences in WBC numbers between samples)
- Stabilize RNA in whole blood When collecting blood samples for RNA analysis, storage of the samples prior to RNA isolation is often required
- The RiboPure™-Blood Kit contains RNA later ™ Solution for the stabilization of RNA in whole blood
- It also “freezes” the gene expression profile of the cells
- Treated samples can be safely stored at ambient temperature for extended periods of time (up to three days or more)
- Blood samples stored in RNA later ™ Solution yield RNA of comparable quality to blood samples processed directly (see figure)
- Simple procedure yields highly pure RNA The RiboPure™-Blood procedure consists of three steps: 1) addition of Lysis Solution directly to RNA later ™ -treated or fresh whole blood, 2) initial RNA purification by phenol/chloroform extraction, and 3) final RNA purification on a glass fiber filter
- An additional DNase treatment can then be performed with the DNA- free™ Reagents included in the kit
- The entire procedure, including DNase treatment, can be completed in less than 1 hour
- Accessory products The RNA can subsequently be enriched for white blood cell (WBC) mRNA using our GLOBINclear™ Kits to selectively remove globin transcripts
- The Mouse RiboPure™-Blood RNA Isolation Kit (Cat
- No
- AM0951) is also available for the recovery of total RNA—and optionally microRNA—directly from mouse or rat blood
- Mouse RiboPure™-Blood includes sample tubes preloaded with RNA later ™ Solution to prevent RNA degradation and preserve RNA profiles.