18-010-017

Invitrogen™ DNA Polymerase I

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 18-010-017-Each-of-1 In Stock ₹ 17,454.24

18-010-017 - Each of 1

₹ 17,454.24

In Stock

Quantity

1

Base Price: ₹ 17,454.24

GST (18%): ₹ 3,141.763

Total Price: ₹ 20,596.003

Content And Storage

Store in freezer (-5 to -30°C).

Form

Liquid

Exonuclease Activity

3' - 5', 5' - 3'

Shipping Condition

Wet Ice

Description

DNA Polymerase

Polymerase

DNA Polymerase I

Quantity

250 U

Hot Start

No

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Description

  • Performance and quality testing Endodeoxyribonuclease assay; efficiency of DNase I-dependent nick translation determined
  • Unit definition One unit incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min at 37°C using template/primer
  • Unit reaction conditions 50mM potassium phosphate (pH 7.0), 6.7mM MgCl 2 , 1mM 2-mercaptoethanol, 80μg/mL template/primer, 32μM dTTP, 69nM [ 3H ]dTTP, and enzyme in 100μL for 30 min at 37°C
  • Also incorporates biotinylated nucleotides
  • Applications: DNase I-dependent nick translation, second-strand synthesis in cDNA cloning, fill-in of 5' overhangs Source: purified from E
  • coli expressing the DNA Polymerase I gene on a plasmid Cloning, Restriction Enzyme Cloning Order Info Shipping Condition: Wet Ice