4921658
Invitrogen™ DNA Polymerase I
DNA polymerase with 5'→3' and 3'→5' exodeoxyribonuclease activities
Manufacturer: Fischer Scientific
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Description
DNA Polymerase
Exonuclease Activity
3' - 5', 5' - 3'
Hot Start
No
Polymerase
DNA Polymerase I
Shipping Condition
Wet Ice
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Description
- Performance and quality testing Endodeoxyribonuclease assay; efficiency of DNase I-dependent nick translation determined
- Unit definition One unit incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min at 37°C using template/primer
- Unit reaction conditions 50mM potassium phosphate (pH 7.0), 6.7mM MgCl 2 , 1mM 2-mercaptoethanol, 80μg/mL template/primer, 32μM dTTP, 69nM [ 3H ]dTTP, and enzyme in 100μL for 30 min at 37°C
- Also incorporates biotinylated nucleotides
- Applications: DNase I-dependent nick translation, second-strand synthesis in cDNA cloning, fill-in of 5' overhangs Source: purified from E
- coli expressing the DNA Polymerase I gene on a plasmid Cloning, Restriction Enzyme Cloning Order Info Shipping Condition: Wet Ice