4919378

Invitrogen™ AccuPrime™TaqDNA Polymerase System

The AccuPrimeTaqDNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases.

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Detection Method

Primer-probe

PCR Method

1-step RT-qPCR, Multiplex qPCR

Reaction Speed

Standard

Shipping Condition

Wet or Dry Ice

Concentration

10X

Hot Start

Built-In Hot Start

Reaction Format

Separate Components

GC-Rich PCR Performance

Low

Polymerase

AccuPrime Taq DNA Polymerase

Product Type

DNA Polymerase System

For Use With (Application)

Hot-start PCR

Fidelity (vs. Taq)

2X

Overhang

3'-A

Size (Final Product)

5 kb or less

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Description

  • The AccuPrime Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases
  • Platinum anti- Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR
  • This combination improves the fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR
  • AccuPrime Taq DNA Polymerase broadens primer annealing temperatures, giving you optimal performance between 55°C and 65°C
  • AccuPrime Taq DNA Polymerase features Control mispriming at every cycle for single-band PCR products Minimal reaction optimization and primer set redesign Reaction assembly at room temperature Applications AccuPrime Taq DNA Polymerase can be used for a wide variety of applications, including: Multiplex PCR TOPO TA Cloning Allele-specific amplifications High-throughput screening AccuPrime buffers The 10X AccuPrime buffers contain thermostable AccuPrime protein, Mg ++ , and deoxyribonucleotide triphosphates at concentrations sufficient to allow amplification during PCR
  • Two individual buffer systems (10X AccuPrime PCR Buffer I and II) are provided for amplification of specific types of templates
  • 10X AccuPrime PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids
  • 10X AccuPrime PCR Buffer II is designed for genomic DNA (200 bp–4 kb)
  • Order Info Shipping Condition: Approved for shipment on wet or dry ice