4922147

Invitrogen™ AccuPrime™TaqDNA Polymerase, High Fidelity

Invitrogen AccuPrimeTaqDNA Polymerase, High Fidelity, provides qualified reagents for the high-fidelity PCR amplification of nucleic acid templates.

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Detection Method

Primer-probe

Polymerase

AccuPrime Taq DNA Polymerase High Fidelity

Product Type

DNA Polymerase

For Use With (Application)

Hot-start PCR, High-fidelity PCR

Hot Start

Built-In Hot Start

Size (Final Product)

20 kb or less

GC-Rich PCR Performance

Low

Reaction Speed

Standard

Shipping Condition

Dry Ice

Fidelity (vs. Taq)

9X

Reaction Format

Separate Components

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Description

  • Invitrogen AccuPrime Taq DNA Polymerase, High Fidelity, provides qualified reagents for the high-fidelity PCR amplification of nucleic acid templates
  • It includes an enzyme blend of Platinum Taq DNA Polymerase and a proofreading enzyme, plus AccuPrime accessory proteins for improved PCR fidelity, yield, and specificity over other hot-start DNA polymerases
  • Features of AccuPrime Taq DNA Polymerase, High Fidelity High specificity and yield for robust PCR amplification 9-fold higher fidelity than Taq DNA polymerase alone Mimimal optimization steps, even with non-optimized primer sets Efficient amplification of targets over a broad size range up to 20 kb How it works Pyrococcus species GB -D polymerase is a proofreading enzyme that possesses a 3' to 5' exonuclease activity
  • Mixing this enzyme with Taq DNA Polymerase increases fidelity and allows amplification of simple and complex DNA templates over a large range of target sizes
  • The Platinum antibody complexes with Taq DNA polymerase and inhibits activity at room temperature
  • Activity is restored after the initial denaturation step, providing an automatic hot start PCR
  • The thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, preventing mispriming and enhancing PCR specificity and yield
  • Order Info Shipping Condition: Dry ice