4926955
Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase
AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content.
Manufacturer: Fischer Scientific
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GC-Rich PCR Performance
High
Reaction Speed
Standard
For Use With (Application)
Hot-start PCR
Hot Start
Built-In Hot Start
Reaction Format
Separate Components
Polymerase
AccuPrime GC-Rich DNA Polymerase
Shipping Condition
Wet or Dry Ice
Fidelity (vs. Taq)
2X
Overhang
Blunt
Size (Final Product)
5 kb or less
Related Products
Description
- AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content
- The kit offers a choice of buffers for amplifying genomic DNA targets (Buffer A) or non-GC-rich cDNA, plasmid, and lambda-based targets (Buffer B)
- AccuPrime GC-Rich DNA Polymerase features High yields for targets up to 5 kb in length AccuPrime accessory proteins for improved PCR specificity Sensitivity down to 5 ng of template DNA Robust and specific amplifications The AccuPrime buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction
- The polymerase itself, from the archaebacterium Pyrolobus fumarius , has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C
- Unit definition One unit of AccuPrime GC-Rich DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble material in 30 min
- at 74°C
- Order Info Approved for shipment on Wet or Dry Ice