Thermo Scientific™ Pierce™ Antibody Clean-up Kit

Description

• Thermo Scientific™ Pierce Antibody Clean-Up Kit is for removing BSA, gelatin and other stabilizer proteins from antibody stock solutions before labeling and conjugation procedures
• This is a complete and easy-to-use kit for removing BSA and gelatin stabilizing proteins (up to 1%) from IgG samples
• The protocol uses Thermo Scientific Melon™ Gel to bind these frequently used storage proteins, allowing the antibody to flow through in a purified form
• The antibody is recovered in an amine-free and carboxyl-free buffer, ready for use in downstream applications such as biotinylation, fluorescent labeling, crosslinking, immobilization and enzymatic fragmentation (e.g., Fab preparation)
• Bovine serum albumin (BSA) and gelatin are often added to purified antibody solutions as stabilizers for long-term storage
• Inclusion of 0.2 to 1% (2 to 10mg/mL) BSA or gelatin helps stabilize dilute antibody solutions
• Generally these added proteins do not interfere with immunodetection methods; however, their presence in solution does interfere with amine-reactive conjugation chemistries that are the basis for most biotinylation, fluorescent dye labeling, antibody fragmentation and covalent immobilization methods
• For such methods, the antibody must be isolated from the stabilizing proteins, and the Antibody Clean-up Kit accomplishes this efficiently
• Highlights: Fast – removes carrier protein from antibody solutions in half the time required for purifying antibodies through Protein A or G purification procedures Gentle – no need to neutralize or desalt to mitigate the effects of harsh elution buffers Complete – supplied desalting columns provide for easy buffer exchange into the required purification buffer or quick exchange into final buffer of choice Economical – sufficient reagents to perform 10 antibody clean-up reactions Includes: Melon Gel IgG Purification Support and Buffer, desalting columns, microcentrifuge spin columns and tubes