Thermo Scientific™ Pierce™ Fab Preparation Kit

Description

• Thermo Scientific™ Pierce Fab Preparation Kits use immobilized papain protease to digest human or mouse IgG antibodies to make separate Fab and Fc fragments and subsequently to purify the Fab using Protein A agarose
• These kits use papain, a nonspecific thiol-endopeptidase, to enzymatically cleave whole IgG just above the hinge region to create two separate Fab fragments and one Fc fragment per antibody molecule
• Because the papain protease is supplied in immobilized form as beaded agarose resin, the digestion reaction is easily stopped by removing the resin from the IgG solution; the result is digest products that are enzyme-free
• The antibody digestion reaction is performed in convenient disposable spin columns that allow efficient removal of the immobilized protease and maximum recovery of the IgG fragments
• Also included in the kits are Protein A spin columns and buffers to efficiently purify the resulting fragments
• Protein A binds the Fc fragments and undigested IgG, allowing the pure Fab fragments to be recovered in the flow-through fraction
• Thermo Scientific Zeba™ Desalting Spin Columns make IgG-sample preparation quick and easy
• Highlights: Enzyme-free digestion products – immobilized papain (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the protease Suitable for human and other species of Ig G – the kit procedure is optimized for human, mouse and rabbit IgG, but papain-based digestion is effective for many species and subclasses of IgG including rat, goat and pig (Note: for best results with mouse IgG 1 , use Part No
• 44980) High capacity – kit provides for digestion and purification from as much as 4mg of IgG at a time
• Use the standard size kit for fragmenting 0.25 to 4mg IgG, and the micro kit for 25 to 250μg IgG Provides ready-to-use Fab – digestion and final recovery of purified Fab fragments occurs in neutral pH sodium phosphate buffer, suitable for storage or immediate use in typical applications Complete and easy to use – kits include all reagents, spin columns and accessories needed to prepare and purify antibody fragments Includes: Papain agarose resin, cysteine, digestion buffer, Protein A columns with binding and elution buffers, spin desalting columns and accessories