Thermo Scientific™ Pierce™ F(ab')2 Preparation Kit

Description

• Thermo Scientific™ Pierce F(ab') 2 Preparation Kits use immobilized pepsin protease to selectively digest whole human and other IgG antibodies to make F(ab') 2 fragments that retain antigen binding activity
• These kits use pepsin, a nonspecific endopeptidase, to enzymatically digest the Fc portion of whole IgG to yield the fragment known as F(ab') 2
• This fragment is composed of a pair of Fab' units connected by two disulfide bonds
• Because the pepsin protease is supplied in immobilized form as beaded agarose resin, the digestion reaction is easily stopped by removing the resin from the IgG solution; the result is digest products that are enzyme-free
• An optimized procedure, high-performance desalting and Protein A spin columns make these kits easy to use
• Highlights: Enzyme-free digestion products – immobilized pepsin (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the protease Suitable for human and other species of IgG – the kit procedure is optimized for human, and rabbit, but pepsin-based digestion is effective for other species and subclasses of IgG (Note: for best results with mouse IgG 1 , use Part No
• 44980) High capacity – kit provides for digestion and purification from as much as 4mg of IgG at a time
• Use the standard size kit for fragmenting 0.25 to 4mg IgG, and the micro kit for 25 to 250μg IgG Complete and easy to use – kits include all reagents, spin columns and accessories needed to prepare and purify antibody fragments Includes: Pepsin agarose resin, digestion buffer, Protein A columns with binding and elution buffers, spin desalting columns and accessories