18-288-019
Invitrogen™ MAX Efficiency™ DH5αF'IQ Competent Cells
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 18-288-019-Each-of-1 | In Stock | ₹ 29,860.44 |
18-288-019 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 29,860.44
GST (18%): ₹ 5,374.879
Total Price: ₹ 35,235.319
Product Type
Chemically Competent Cells
Improves Plasmid Quality
Yes (endA1)
Transformation Efficiency Level
Medium Efficiency (1 x 108to 1 x 109cfu/μg)
Antibiotic Resistance Bacterial
Yes (Kanamycin)
Blue/White Screening
Yes (lacZΔM15)
Preparing Unmethylated DNA
No
Shipping Condition
Dry Ice
Species
E. coli (K12)
Format
Tube
Quantity
5 x 200 μL
Contains F' Episome
Yes
Cloning Methylated DNA
No
Content And Storage
• MAX Efficiency DH5αF'IQ Competent Cells (5 x 200 μL)• F'IQ Lawn Cell Assy (2 x 1.5 mL)• M13mp18 RF DNA (1 vial)Store at –80°C.• S.O.C. Medium (2 x 6 mL)Store S.O.C. Medium at 4°C or room temperature.
Cloning Unstable DNA
Not suitable for cloning unstable DNA
High-throughput Compatibility
Low
Reduces Recombination
Yes (recA1)
T1 Phage - Resistant (tonA)
No
Promoter
Laclq
Product Line
MAX Efficiency™
Related Products
Description
- MAX Efficiency DH5αF´IQ Competent Cells are high transformation efficiency chemically competent cells prepared by a patented modification of the procedure of Hanahan
- This strain is a derivative of the DH5α strain, sharing the same benefits
- The DH5αF´IQ strain contains an F´ episome and is a host for M13mp cloning vectors
- It also supports cloning plasmids with an f1-like origin of replication (phagemids) or vector that uses expression from the lac promoter
- DH5αF´IQ cells can yield >1 x 10 8 plaque forming units/μg M13mp19 RF with non-saturating amounts (5 pg) of DNA
- MAX Efficiency DH5αF´IQ Competent Cells have the lac ZΔM15 genotype, allowing blue-white screening on plates containing either X-Gal or Bluo-Gal
- They also carry the rec A1 mutation which helps to reduce the rate of recombination while propagating plasmid DNA and the end A1 mutation that increases plasmid DNA yield and quantity
- MAX Efficiency DH5αF´IQ Competent Cells offer: • >3 x 10 8 transformants/μg efficiency • >1 × 10 8 plaque forming units/μg M13mp19 RF • Stable F´ episome that eliminates need for growth on minimal media • lac ZΔM15 marker that provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-Gal or Bluo-Gal • Plasmid DNA maintenance with minimum recombination events due rec A1 mutation • end A1 mutation that enables increased plasmid yield and quantity Note: DH5αF'IQ lawn cells are provided to allow for plaque formation and a vial of monomer M13mp18 RF DNA are supplied as a control
- Genotype F – φ80 lac ZΔM15 Δ( lac ZYA- arg F)U169 rec A1 end A1 hsd R17 (r K –, m K +) pho A sup E44 λ – thi – 1 gyr A96 rel A1/F′ [ pro AB+ lac IqZΔM15 zzf :: Tn 5(KmR)] Find the strain and format that fits your needs • The DH5α strain and its derivatives are available with a variety of transformation efficiencies and in both electrocompetent and chemically competent formats
- • DH5α-T1 R competent cells are available in MultiShot format for high throughput applications
- Order Info Shipping Condition: Dry Ice