C66411
Invitrogen™ TOP10 Electrocomp™ Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | C66411-Each-of-1 | In Stock | ₹ 46,031.28 |
C66411 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 46,031.28
GST (18%): ₹ 8,285.63
Total Price: ₹ 54,316.91
Product Type
Electrocompetent Cells
Improves Plasmid Quality
Yes (endA1)
Transformation Efficiency Level
High Efficiency (>1 x 1010cfu/μg)
Antibiotic Resistance Bacterial
Yes (Streptomycin)
Blue/White Screening
Yes (lacZΔM15)
Plasmid
May be used for plasmids >20 kb
Reduces Recombination
Yes (recA1)
T1 Phage - Resistant (tonA)
No
Format
Tube
Quantity
10 x 100 μL
Contains F' Episome
No
Cloning Methylated DNA
Yes (mcrA)
Content And Storage
• 10 x 100 μL TOP10 ElectrocompE. coli• 50 μL pUC19 vector (10 pg/μL)Store at –80°C.• 15 mL S.O.C. MediumStore at room temperature.
Cloning Unstable DNA
Not suitable for cloning unstable DNA
High-throughput Compatibility
Low
Preparing Unmethylated DNA
No
Shipping Condition
Dry Ice
Species
E. coli (K12)
Product Line
Electrocomp™
Related Products
Description
- TOP10 Electrocomp E
- coli cells are provided at a transformation efficiency of 1 x 10 10 cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation
- This strain is used in wide range of applications and allows stable replication of high-copy number plasmids and maintenance of large plasmids
- The TOP10 genotype is similar to the DH10B strain and features: • hsd R for efficient transformation of unmethylated DNA from PCR amplifications • mcr A for efficient transformation of methylated DNA from genomic preparations • lac ZΔM15 for blue/white color screening of recombinant clones • end A1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by Endonuclease I • rec A1 for reduced occurrence of non-specific recombination in cloned DNA Notes Please see the product manual for reaction scale recommendations
- A high-voltage electroporation apparatus is required
- Genotype F - mcr A Δ( mrr - hsd RMS- mcr BC ) φ80 lac ZΔM15 Δ lac X74 rec A1 ara D139 Δ( ara - leu )7697 gal U gal K rps L end A1 nup G Find the strain and format that fits your needs • TOP10 is available in chemically competent and electrocompetent cell formats
- • Choose MultiShot competent cells for high-throughput cloning applications.