Description

• General description: Native endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins and the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK.
• Warning: Toxicity: Harmful (C)
• Unit Definition: One unit is defined as the amount of enzyme that will release a sufficient amount of azo dye from AZOCOLL Substrate (Cat. No. 194932) to produce an increase in absorbance at 520 nm of 0.001 per min at 37°C, pH 7.5.
• Physical form: Lyophilized from 10 mM Tris-HCl, pH 7.5.
• Reconstitution: Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
• Other Notes: Drapeau, G.R. 1980. J. Biol. Chem.255, 839.
• Legal Information: CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany