F8435
PNGase F from Elizabethkingia meningoseptica
lyophilized powder, recombinant, expressed in E. coli
Manufacturer: Sigma Aldrich
CAS Number: 83534-39-8
Synonym(S): N-Glycosidase F, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum, Peptide N-glycosidase
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 50 UNITS | F8435-50-UNITS | In Stock | ₹ 28,427.10 |
| 300 UNITS | F8435-300-UNITS | In Stock | ₹ 1,39,427.10 |
F8435 - 50 UNITS
In Stock
Quantity
1
Base Price: ₹ 28,427.10
GST (18%): ₹ 5,116.878
Total Price: ₹ 33,543.978
recombinant
expressed in E. coli
Quality Level
200
conjugate
(N-linked)
grade
Proteomics Grade
form
lyophilized powder
specific activity
≥1,000 U/mg
shelf life
≥1 weeks at 2‑8 °C (for reconstituted solution)≥1 yr at -20 °C
mol wt
~36 kDa
shipped in
wet ice
storage temp.
−20°C
Other Options
| Image | Product Name | Manufacturer | Price Range | |
|---|---|---|---|---|
 | PNGase F from Elizabethkingia meningoseptica | -- | ₹ 37,518.00 - ₹ 1,82,739.30 | |
| | PNGase F from Elizabethkingia miricola | -- | ₹ 34,720.80 - ₹ 59,584.80 | |
| | N-Glycosidase F, Elizabethkingia meningosepticum | -- | ₹ 42,350.00 | |
| | PNGase F from Elizabethkingia meningoseptica | -- | ₹ 78,510.30 | |
| | Glycopeptidase A from almonds | -- | ₹ 1,06,038.30 | |
| | N-Glycosidase F, Chryseobacterium meningosepticum, Recombinant, E. coli | -- | ₹ 59,040.00 | |
| | PNGase F from Elizabethkingia meningoseptica | -- | ₹ 28,726.80 - ₹ 1,43,278.80 | |
| | 83534-39-8 | PNGase F | -- | -- |
Related Products
Description
- Application: Highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-teminal 6x histidine fusion tag.
- Biochem/physiol Actions: Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.
- Packaging: PNGase F was used for deglycosylation of P-glycoprotein in a study to investigate the dual impact of statins on p-glycoprotein and its effect on doxorubicin cytotoxicity in human neuroblastoma cells.[1] It was used to treat a purified protein, mouse cone ultraviolet (MUV) pigment, before use in quantitative immunoblot analysis in a study[2] It is used to deglycosylate N-linked glycoproteins. Highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-teminal 6x histidine fusion tag.
- Unit Definition: One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.
SAFETY INFORMATION
Pictograms
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable