P7367

PNGase F from Elizabethkingia meningoseptica

BioReagent, ≥95% (SDS-PAGE), for proteomics

Manufacturer: Sigma Aldrich

CAS Number: 83534-39-8

Synonym(S): N-Glycosidase F, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum, Peptide N-glycosidase

Select a Size

Pack Size SKU Availability Price
50 UNITS P7367-50-UNITS In Stock ₹ 37,518.00
300 UNITS P7367-300-UNITS In Stock ₹ 1,82,739.30

P7367 - 50 UNITS

₹ 37,518.00

In Stock

Quantity

1

Base Price: ₹ 37,518.00

GST (18%): ₹ 6,753.24

Total Price: ₹ 44,271.24

biological source

bacterial (Elizabethkingia meningoseptica)

Quality Level

200

conjugate

(N-linked)

grade

for proteomics

product line

BioReagent

Assay

≥95% (SDS-PAGE)

form

powder

shelf life

≥1 weeks at 2‑8 °C (for a reconstituted solution >500 units/ml)≥1 yr at 2‑8 °CSolution is stable for at least 3 freeze-thaw cycles

mol wt

~36 kDa

concentration

≥300 units/mL≥50 units/mL

Other Options

Image Product Name Manufacturer Price Range
PNGase F from Elizabethkingia miricola
-- ₹ 34,720.80 - ₹ 59,584.80
N-Glycosidase F, Elizabethkingia meningosepticum
-- ₹ 42,350.00
PNGase F from Elizabethkingia meningoseptica
-- ₹ 78,510.30
Glycopeptidase A from almonds
-- ₹ 1,06,038.30
N-Glycosidase F, Chryseobacterium meningosepticum, Recombinant, E. coli
-- ₹ 59,040.00
PNGase F from Elizabethkingia meningoseptica
-- ₹ 28,726.80 - ₹ 1,43,278.80
PNGase F from Elizabethkingia meningoseptica
-- ₹ 28,427.10 - ₹ 1,39,427.10
83534-39-8 | PNGase F
-- --

Related Products

Img

Sigma Aldrich

F8435

lyophilized powder, recombinant, ex...

Img

Sigma Aldrich

362185

--

Img

Sigma Aldrich

G5166

buffered aqueous solution...

Img

Sigma Aldrich

EMS0005

Dimethlyated, Proteomics Grade, rec...

Img

Sigma Aldrich

A4551

lyophilized powder, 500-1,500 units...

Img

Sigma Aldrich

SAE0090

≥300 units/mg protein, ST6GAL1 huma...

Img

Sigma Aldrich

D5319

recombinant, expressed in Pichia pa...

Img

Sigma Aldrich

A6380

Type II-A, lyophilized powder, ≥1,5...

Description

  • General description: Proteomics Grade PNGase F has been extensively purified and lyophilized from dilute potassium phosphate buffer to produce a stable product. The product is free from glycerol and other stabilizers, and contains very low levels of buffer salts. This highly purified material is excellent for N-linked deglycosylation of glycoproteins or glycopeptides in gel, in solution, or on blot membranes.
  • Application: PNGase F from Elizabethkingia meningoseptica has been used:for de-N-glycosylation of Zika20virus E protein[1]to evaluate coxsackievirus and adenovirus receptor glycosylation using CAR-expressing COS cells[2]to verify the N-linked glycosylation of MHC class 1 polypeptide-related sequence A (MICA)[3]
  • Biochem/physiol Actions: Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.
  • Unit Definition: One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

SAFETY INFORMATION

Pictograms

GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves