G1549
PNGase F from Elizabethkingia meningoseptica
ready-to-use solution, recombinant, expressed in E. coli
Manufacturer: Sigma Aldrich
CAS Number: 83534-39-8
Synonym(S): PNGase F from Elizabethkingia meningoseptica, N-Glycosidase F, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum, Peptide N-glycosidase
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 50 UNITS | G1549-50-UNITS | In Stock | ₹ 28,726.80 |
| 300 UNITS | G1549-300-UNITS | In Stock | ₹ 1,43,278.80 |
G1549 - 50 UNITS
In Stock
Quantity
1
Base Price: ₹ 28,726.80
GST (18%): ₹ 5,170.824
Total Price: ₹ 33,897.624
recombinant
expressed in E. coli
Quality Level
200
conjugate
(N-linked)
grade
Proteomics Grade
form
ready-to-use solution
specific activity
≥1000 U/mg
shelf life
≥1 yr at -20 °C
mol wt
~36 kDa
shipped in
wet ice
storage temp.
−20°C
Other Options
| Image | Product Name | Manufacturer | Price Range | |
|---|---|---|---|---|
 | PNGase F from Elizabethkingia meningoseptica | -- | ₹ 37,518.00 - ₹ 1,82,739.30 | |
| | PNGase F from Elizabethkingia miricola | -- | ₹ 34,720.80 - ₹ 59,584.80 | |
| | N-Glycosidase F, Elizabethkingia meningosepticum | -- | ₹ 42,350.00 | |
| | PNGase F from Elizabethkingia meningoseptica | -- | ₹ 78,510.30 | |
| | Glycopeptidase A from almonds | -- | ₹ 1,06,038.30 | |
| | N-Glycosidase F, Chryseobacterium meningosepticum, Recombinant, E. coli | -- | ₹ 59,040.00 | |
| | PNGase F from Elizabethkingia meningoseptica | -- | ₹ 28,427.10 - ₹ 1,39,427.10 | |
| | 83534-39-8 | PNGase F | -- | -- |
Related Products
Description
- Application: Recombinant PNGase F has been purified by affinity chromatography and dialyzed into a 50% glycerol solution with 10 mM potassium phoosphate pH 7.5 to produce a stable product. The product contains low levels of buffer salts. This highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-terminal 6x histidine fusion tag. PNGase F from Elizabethkingia meningoseptica has been used in deglycosylation assay in human plasma samples[1] and in deglycosylation of chondroitin sulfate proteoglycan.[2]
- Biochem/physiol Actions: PNGase F from Elizabethkingia meningoseptica has glycan-binding catalytic domain and a bowl-like domain at the N-terminus. It cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.[3] It is cost-effectively produced on a large scale in prokaryotic hosts and requires divalent zinc ions for its enzymatic activity.[4]
- Unit Definition: One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.
- Physical form: Supplied as 300 Units/mL enzyme in 50% (v/v) glycerol and 50% (v/v) 20 mM Potassium Phosphate, pH 7.5.
SAFETY INFORMATION
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable