N3254
4-Nitrophenyl phosphate di(tris) salt
phosphatase substrate, ≥97% (enzymatic), powder
Manufacturer: Sigma Aldrich
CAS Number: 68189-42-4
Synonym(S): 4-Nitrophenyl phosphate bis(tris) salt
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 G | N3254-1-G | In Stock | ₹ 4,178.45 |
| 5 G | N3254-5-G | In Stock | ₹ 10,608.50 |
| 25 G | N3254-25-G | In Stock | ₹ 39,933.43 |
| 100 G | N3254-100-G | In Stock | ₹ 1,08,022.68 |
N3254 - 1 G
In Stock
Quantity
1
Base Price: ₹ 4,178.45
GST (18%): ₹ 752.121
Total Price: ₹ 4,930.571
product name
4-Nitrophenyl phosphate di(tris) salt, phosphatase substrate
Quality Level
100
Assay
≥97% (enzymatic)
form
powder
solubility
water: 50 mg/mL, clear, colorless to light yellow
storage temp.
−20°C
SMILES string
NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O
InChI
1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2
InChI key
XXAXKCWOTRABOW-UHFFFAOYSA-N
Other Options
| Image | Product Name | Manufacturer | Price Range | |
|---|---|---|---|---|
 | Sigma Aldrich Fine Chemicals Biosciences 4-Nitrophenyl phosphate di(tris) salt phosphatase substrate | 68189-42-4 | MFCD00037009 | 100g | -- | ₹ 1,02,980.02 | |
| | Chem-Impex International, Inc. 4-Nitrophenyl phosphate di(tris) salt | 68189-42-4 | MFCD00037009 | 100G | -- | ₹ 17,454.24 | |
| | Chem-Impex International, Inc. 4-Nitrophenyl phosphate di(tris) salt | 68189-42-4 | MFCD00037009 | 5G | -- | ₹ 3,358.23 | |
| | Sigma Aldrich Fine Chemicals Biosciences 4-Nitrophenyl phosphate di(tris) salt phosphatase substrate | 68189-42-4 | MFCD00037009 | 5G | -- | ₹ 10,407.52 | |
| | 4-Nitrophenyl phosphate ditromethamine | -- | ₹ 7,614.84 | |
| | 68189-42-4 | 4-Nitrophenyl phosphate di(tris) salt | -- | ₹ 2,053.44 - ₹ 1,12,340.28 |
Related Products
Description
- Application: 4-Nitrophenyl phosphate di(tris) salt has been used:as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysates[1]to determine phosphatase activity using native polyacrylamide gel electrophoresis (PAGE)[2]for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA)[2]
- Biochem/physiol Actions: p-Nitrophenyl Phosphate (pNPP) is a preferred substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures, because of its high sensitivity. This substrate produces a soluble yellow end-product and can be read spectrophotometrically at 405 nm.[3]
SAFETY INFORMATION
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves