P4252
Phosphatase, Alkaline from Escherichia coli
ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)
Manufacturer: Sigma Aldrich
CAS Number: 9001-78-9
Synonym(S): Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 UNITS | P4252-100-UNITS | In Stock | ₹ 21,056.70 |
| 200 UNITS | P4252-200-UNITS | In Stock | ₹ 35,042.70 |
| 1000 UNITS | P4252-1000-UNITS | In Stock | ₹ 1,18,381.50 |
P4252 - 100 UNITS
In Stock
Quantity
1
Base Price: ₹ 21,056.70
GST (18%): ₹ 3,790.206
Total Price: ₹ 24,846.906
biological source
Escherichia coli
Quality Level
300
form
ammonium sulfate suspension
specific activity
30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)
storage temp.
2-8°C
Other Options
Related Products
Description
- Application: Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4252 has been used for bulk digestion of tRNA [1]. Product P4252 is provided in the ratio of 30-90 units/mg of protein.
- Biochem/physiol Actions: Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.
- Caution: Presence of 0.1% SDS in the alkaline phosphatase assays will inhibit any nuclease contamination in the sample.
- Unit Definition: One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 10.4 at 37 °C.
- Physical form: Suspension in 2.5 M (NH4)2SO4
- Analysis Note: The activity at pH 8.0 at 25 °C is approximately the same as at pH 10.4 at 37 °C.
SAFETY INFORMATION
Pictograms
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves