Thermo Scientific™ GeneBLAzer™ PPAR delta DA Assay Kit
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1395-Each-of-1 | In Stock | ₹ 1,31,762.40 |
K1395 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,31,762.40
GST (18%): ₹ 23,717.232
Total Price: ₹ 1,55,479.632
Alternate Agonists
Estrone, Genistein, Diethylstibestrol, Ethynylestradiol
Assay Entry
Cell-based beta lactamase reporter gene
Content And Storage
Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate:• PPAR delta HEK 293T DA cells (K1395A)• LiveBLAzer™-FRET B/G Loading Kit, 70 μgShipping:Dry IceStorage:-80°C (liquid nitrogen)
Gene ID (Entrez)
5467
Primary Agonist
17-β-estradiol
Quantity
1 plate
Target Entry
PPAR delta, NR1C2, PPARD
Cell Line
293T (HEK)
Druggable Target
Nuclear Receptors
Antagonists
4-OH-tam+0.2 nM E2, Tam+0.2 nM E2, 4-OH-tamoxifen, Tamoxifen, RU486+0.2 nM E2, RU486
Cell State
Division-Arrested Cells
Detection Method
Fluorescence
Gene Symbol
PPARδ
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Type
Division-Arrested Cells Assay Kit
System Type
GeneBLAzer™
Related Products
Description
- GeneBLAzer™ PPAR delta DA (Division Arrested) cells and PPAR delta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Peroxisome Proliferator-Activated Receptor-delta (PPAR delta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™ UAS-bla HEK 293T cell line
- GeneBLAzer™ UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS)
- When an agonist binds to the LBD of the GAL4 (DBD)-PPAR delta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase
- Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates
- DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction
- Both PPAR delta DA cells and PPAR delta-UAS-bla HEK 293T cells are functionally validated for Z' and EC 50 concentrations of L-165041
- In addition, PPAR delta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request)
- Additional testing data using alternate stimuli are also available.