K1407
Thermo Scientific™ GeneBLAzer™ RAR-beta DA Assay Kit
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1407-Each-of-1 | In Stock | ₹ 1,26,457.68 |
K1407 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,26,457.68
GST (18%): ₹ 22,762.382
Total Price: ₹ 1,49,220.062
Alternate Agonists
Docosahexaenoic Acid, Phytanic Acid, All-trans-retinoic acid
Assay Entry
Cell-based beta lactamase reporter gene
Content And Storage
Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate:• RAR beta HEK 293T DA cells (K1407A)• LiveBLAzer™-FRET B/G Loading Kit, 70 μgShipping:Dry IceStorage:-80°C (liquid nitrogen)
Gene Symbol
RARβ
Quantity
1 plate
Target Entry
RAR beta, NR1B2, RARB
Cell Line
293T (HEK)
Druggable Target
Nuclear Receptors
Antagonists
none
Cell State
Division-Arrested Cells
Detection Method
Fluorescence
Primary Agonist
9-cis-retinoic acid
Reporter Gene
BLA (Beta-Lactamase)
Type
Division-Arrested Cells Assay Kit
System Type
GeneBLAzer™
Related Products
Description
- GeneBLAzer™RAR beta DA (Division Arrested) cells and RAR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor beta (RAR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line
- GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS)
- When an agonist binds to the LBD of the GAL4 (DBD)-RAR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase
- DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction
- Both RAR beta DA cells and RAR beta-UAS-bla HEK 293T cells are functionally validated for Z' and EC 50 concentrations of all-trans retinoic acid (ATRA)
- In addition, RAR beta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.