K1389
Thermo Scientific™ GeneBLAzer™ TR beta DA Assay Kit
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1389-Each-of-1 | In Stock | ₹ 1,30,222.32 |
K1389 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,30,222.32
GST (18%): ₹ 23,440.018
Total Price: ₹ 1,53,662.338
Alternate Agonists
9-cis-retinoic acid, AM580
Assay Entry
Cell-based beta lactamase reporter gene
Content And Storage
Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate:• TR beta HEK 293T DA cells (K1389A)• LiveBLAzer™-FRET B/G Loading Kit, 70 μgShipping:Dry IceStorage:-80°C (liquid nitrogen)
Gene ID (Entrez)
7068
Primary Agonist
All-trans-retinoic acid (ATRA)
Quantity
1 plate
Target Entry
T3RB, TR beta, THRB, NR1A2
Cell Line
293T (HEK)
Druggable Target
Nuclear Receptors
Antagonists
RP-41-5253
Cell State
Division-Arrested Cells
Detection Method
Fluorescence
Gene Symbol
TRβ
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Type
Division-Arrested Cells Assay Kit
System Type
GeneBLAzer™
Related Products
Description
- GeneBLAzer™TR beta DA(Division Arrested) cells and TR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Thyroid hormone receptor beta (TR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UASbla HEK 293T cell line
- GeneBLAzer™UAS-bla HEK 293T cells stably express a betalactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS)
- When an agonist binds to the LBD of the GAL4 (DBD)-TR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase
- Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates
- DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction
- Both TR beta DA cells and TR beta-UAS-bla HEK 293T cells are functionally validated for Z' and EC 50 concentrations of T3 Thryoid hormone
- In addition, TR beta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request)
- Additional testing data using alternate stimuli are also available.