K1421
Thermo Scientific™ GeneBLAzer™ ERR Alpha DA Assay Kit
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1421-Each-of-1 | In Stock | ₹ 1,29,366.72 |
K1421 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,29,366.72
GST (18%): ₹ 23,286.01
Total Price: ₹ 1,52,652.73
Alternate Agonists
L165, 041, GW 1929, GW 7647, GW 0742
Assay Entry
Cell-based beta lactamase reporter gene
Content And Storage
Shipping: Dry IceStorage: -80°C/Liquid NitrogenSufficient division-arrested cells and substrate to assay one 384-well plate. Includes:ERR alpha HEK 293 T DA cells (K1421A)LiveBLAzer™-FRET B/G Loading Kit, 70 μg
Gene ID (Entrez)
2101
Primary Agonist
Rosiglitazone
Quantity
1 plate
Target Entry
ESRRA, NR3B1, ERR1, ERR alpha
System Type
GeneBLAzer™
Antagonists
T0070907, GW 9662, GW 6471
Cell State
Division-Arrested Cells
Detection Method
Fluorescence
Gene Symbol
ERRα
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Cell Line
293T (HEK)
Druggable Target
Nuclear Receptors
Related Products
Description
- GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS)
- When an agonist binds to the LBD of the GAL4 (DBD)-ERR alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase
- Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates
- DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction
- Both ERR alpha DA cells and ERR alpha-UAS-bla HEK 293T cells are functionally validated for Z' and EC 50 concentrations of XCT790, an inverse agonist
- In addition, ERR alpha-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request)
- Additional testing data using alternate stimuli are also available.