4526963

Thermo Scientific™ Phusion Blood Direct PCR Kit

Thermo Scientific Phusion Blood Direct PCR Kit is developed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.

Manufacturer: Fischer Scientific

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GC-Rich PCR Performance

High

Polymerase

Phusion Blood II DNA Polymerase

Product Type

Direct PCR Kit

Concentration

2 U/μL

Overhang

Blunt

Sample Type

Blood

PCR Method

Direct PCR

Reaction Speed

Fast

Shipping Condition

Wet or Dry Ice

Hot Start

Built-In Hot Start

Reaction Format

Separate Components

Starting Material

DNA

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Description

  • Thermo Scientific Phusion Blood Direct PCR Kit is for use in the amplification of DNA from whole blood
  • It eliminates the need for a separate DNA purification step prior to PCR
  • The modified Phusion Hot Start II High-Fidelity DNA Polymerase exhibits high resistance to PCR inhibitors found in blood and retains polymerase activity even with samples containing 40% whole blood
  • This DNA polymerase exhibits exceptional accuracy in proofreading and makes the Phusion Blood Direct PCR Kit one of the best choices for demanding applications, such as cloning
  • The Phusion Blood Direct PCR Kit is versatile and permits analysis of blood samples from various species and with different anticoagulants or storage conditions
  • Features No need for time-consuming and expensive DNA purification steps Simple protocol requires minimal hands-on time Extremely short PCR protocol times Modified Phusion Hot Start II High-Fidelity DNA Polymerase delivers abundant yields of specific product Notes The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq -based polymerases.