60-684-050
Invitrogen™ Platinum™TfiExo(-) DNA Polymerase
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 60-684-050-Each-of-1 | In Stock | ₹ 50,309.28 |
60-684-050 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 50,309.28
GST (18%): ₹ 9,055.67
Total Price: ₹ 59,364.95
Content And Storage
Platinum™ Tfi DNA Polymerase (500 units total, at 5U/μL) is supplied with 5X Platinum™ Tfi Reaction Buffer (4 × 1.3 mL) and 50 mM Magnesium Chloride (1 mL). Store at -20°C (non-frost-free).
Polymerase
Tfi DNA Polymerase
Product Line
Platinum™
Quantity
500 units
Concentration
5X
Hot Start
Built-In Hot Start
Reaction Format
Separate Components
Volume
5.2 mL
GC-Rich PCR Performance
Low
Reaction Speed
Standard
Product Type
DNA Polymerase
Shipping Condition
Dry Ice
Fidelity (vs. Taq)
1 X
Overhang
3'-A
Size (Final Product)
1 kb or less
Related Products
Description
- Platinum™ Tfi Exo(-) DNA Polymerase is recombinant Tfi Exo(-) DNA Polymerase complexed with a proprietary antibody mix that inhibits polymerase activity at ambient temperature, allowing room-temperature reaction setup
- Activity is restored after the initial denaturation step in PCR cycling at 94°C, providing an automatic 'hot start' for increased specificity, sensitivity, and yield
- Tfi Exo(-) DNA Polymerase is purified from E
- coli
- expressing cloned mutants of the Thermus filiformis DNA polymerase gene
- This enzyme has 5'→3' polymerase activity, but lacks both 5'→3' and 3'→5' exonuclease activity for improved yield and robustness
- Tfi Exo(-) DNA polymerase is heat-stable and will synthesize DNA at elevated temperatures from single-stranded templates in the presence of a primer
- Applications Platinum™ Tfi Exo(-) DNA Polymerase can be used in protocols that currently use Platinum™ Taq DNA Polymerase without modification
- PCR performance is comparable to that of Platinum™ Taq in yield, specificity, fidelity, and robustness
- Like Platinum™ Taq , Platinum™ Tfi Exo(-) DNA Polymerase has a nontemplate-dependent terminal transferase activity that adds a single deoxyadenosine (A) to the 3' ends of PCR products.