F130WH
Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | F130WH-Each-of-1 | In Stock | ₹ 38,245.32 |
F130WH - Each of 1
In Stock
Quantity
1
Base Price: ₹ 38,245.32
GST (18%): ₹ 6,884.158
Total Price: ₹ 45,129.478
Content And Storage
• Phire Hot Start II DNA Polymerase, 200 μL• 2X Phire Plant Tissue PCR Buffer, 5 x 1.25 mL• Universal Control Primer Mix (25 μM each), 40 μL• Dilution Buffer, 2 x 5 mLStore at -20°C.
PCR Method
Direct PCR
Reaction Speed
Fast
Shipping Condition
Wet or Dry Ice
No. of Reactions
500 Reactions
Reaction Format
Separate Components
Starting Material
DNA
GC-Rich PCR Performance
Medium
Polymerase
Phire Hot Start II DNA Polymerase
Product Type
Direct PCR Kit
Hot Start
Built-In Hot Start
Overhang
Blunt
Sample Type
Plant
Related Products
Description
- Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples
- No DNA purification is required prior to PCR
- The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain
- The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant, fungi, and bacteria
- The Phire Plant Direct PCR Kit includes a complete set of optimized PCR reagents as well as detailed protocols for different sample types, making it an ideal choice for amplification of plant DNA
- In addition, the kit includes control primers for positive control reactions
- The control primers amplify a highly conserved region of chloroplast DNA and are universal for many plant species
- Features No need for time-consuming and expensive DNA purification steps Very little sample material required Two simple protocols for various applications Phire Hot Start II DNA Polymerase provides high yields of specific product with short extension time (20 s/kb) Applications Genotyping Transgene detection Notes The optimal annealing temperature for Phire DNA Polymerase may differ significantly from that of Taq -based polymerases.