Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase & dNTP Mix (10 mM each)

Description

• Thermo Scientific Phusion High-Fidelity DNA Polymerase sets a high standard for high performance PCR
• Featuring an error rate 52-fold lower than that of Taq and 6-fold lower than that of Pfu , Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity
• Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors and generate higher yields with lower enzyme amounts than other DNA polymerase
• Features High fidelity (52X Taq ) Fast PCR due to short extension times (15-30 s/kb) Robust performance, minimal optimization needed High yields of PCR products with minimal enzyme amounts Applications Standard PCR RT-PCR High fidelity and long PCR LAMP, RDA, and MDA cDNA synthesis qPCR DNA labeling and sequencing dNTP Mix Thermo Scientific dNTP Mix contains pre-mixed aqueous solutions of dATP, dCTP, dGTP, and dTTP, each at a final concentration of 10 mM
• The nucleotides have greater than 99% purity and are free of nuclease activities, as well as human and E
• coli DNA
• Mixes offer the possibility of reducing the number of pipetting steps and the risk of reaction setup errors
• dNTP features Stable after multiple freeze-thaw cycles Up to 95% of dNTPs remain in triphosphate form after 7 weeks at room temperature Up to 90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 minute at 94°C, 3 minutes at 72°C) Notes Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).