Thermo Scientific™ Phusion™ Hot Start II DNA Polymerase & dNTP Mix (10 mM each)

Description

• Thermo Scientific Phusion Hot Start II High-Fidelity DNA Polymerase sets a high standard for high performance PCR
• Featuring an error rate 52-fold lower than that of Taq and 6-fold lower than that of Pfu , this polymerase is an excellent choice for cloning and other applications that require high fidelity and offers robust performance with short protocol times, even in the presence of PCR inhibitors
• Phusion Hot Start II High-Fidelity DNA Polymerase generates higher yields with lower enzyme amounts than other DNA polymerases
• With Phusion Hot Start II High-Fidelity DNA Polymerase, amplification proceeds without the production of nonspecific products due to the combination of Phusion DNA Polymerase and a reversibly bound, specific Affibody ligand that inhibits DNA polymerase activity at room temperature
• The Affibody ligand also inhibits the 3' to 5' exonuclease activity of the polymerase, thus preventing degradation of primers and template DNA during reaction setup
• At temperatures that promote polymerase activity, the ligand is released, rendering the polymerase fully active
• Phusion Hot Start II High-Fidelity DNA Polymerase is immediately reactivated at high temperatures and does not require a separate activation step in PCR protocols
• Features High fidelity (52X Taq ) No non-specific amplification and primer degradation during reaction setup Fast PCR due to short extension times (15–30 s/kb) Increased product yields with minimal enzyme amounts Applications Standard PCR qPCR High fidelity and long PCR LAMP-PCR cDNA synthesis RT-PCR, RDA, MDA DNA labeling and sequencing dNTP Mix Thermo Scientific dNTP Mix (Cat
• No
• R0192) contains pre-mixed aqueous solutions of dATP, dCTP, dGTP, and dTTP, each at a final concentration of 10 mM
• The nucleotides have greater than 99% purity and are free of nuclease activities, as well as human and E
• coli DNA
• Mixes offer the possibility of reducing the number of pipetting steps and the risk of reaction setup errors
• Greater than 99% purity confirmed by HPLC Free of human and E
• coli DNA Stable after multiple freeze-thaw cycles Up to 95% of dNTPs remain in triphosphate form after 7 weeks at room temperature Up to 90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 minute at 94°C; 3 minutes at 72°C) Notes This package includes Phusion Hot Start II High-Fidelity DNA Polymerase (4 x 500 U) and high-quality dNTP Mix (10 mM each, 2 x 1 mL)
• Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).