Thermo Scientific™ Pierce™ Thiophilic Adsorption Kit

Description

• Thermo Scientific™ Pierce Thiophilic Adsorbent is a non-proteinaceous immunosorbent resin for gentle preferential binding and purification of immunoglobulins from a variety of sources including mouse, rat, rabbit, goat and human
• Pierce Thiophilic Adsorbent is 6% beaded agarose that has been modified to contain simple sulfone-thioether groups
• The result is an affinity resin that has a high binding capacity and a broad specificity for many species of immunoglobulins when incubated in 0.5M potassium sulfate
• In effect, thiophilic adsorption is a resin-based variation of ammonium sulfate precipitation for quick immunoglobulin purification from crude serum samples
• Unlike antibody fractionation by ammonium sulfate precipitation, purification with thiophilic adsorbent yields concentrated, essentially salt-free, highly purified immunoglobulins at near-neutral pH
• Thus, this simple one-step method eliminates the need for additional treatment of the sample for storage or for subsequent conjugation reactions
• The gentle binding and elution conditions ensure a high protein recovery with excellent preservation of antibody activity
• The resin is available as both a standalone product and as a purification kit containing pre-packed columns and ready-to-use buffers
• Highlights: High capacity – gently bind and purify approx
• 20mg polyclonal antibody per milliliter of adsorbent resin Broad antibody specificity – effective for most immunoglobulin classes and isotypes derived from many different animal species, including chicken IgY Simple and fast – quickly fractionate and purify the immunoglobulin component of serum or ascites samples without subsequent dialysis or desalting steps Gentle – binding condition is nondenaturing and the elution buffer yields concentrated, salt-free immunoglobulin at near neutral pH; provides excellent recovery of functional antibody Includes: Kit contains four Thiophilic Adsorbent Columns (3mL each), ready-to-use binding and elution buffers, column regenerant and storage buffer, and column accessories Recommended for: Mild isolation of immunoglobulins from human serum; Rapid purification of mouse monoclonals from the culture media or ascites fluid; Selective removal of immunoglobulins from fetal calf serum - useful for cell culture in monoclonal antibody production; Rapid, straightforward procedure yielding essentially pure immunoglobulins from crude rabbit serum; Purification of IgY from chicken; Large-scale purification for biotechnology applications