Description

• General description: Aminopeptidase M (APM) is abundantly found in brush border membranes of the kidney, small intestine, placenta, and liver. It is a type II integral membrane protein, known as ectoenzyme.[1]
• Application: Aminopeptidase M, porcine kidney has been used: to test and identify the peaks containing d-residue in the allatotropin-related peptide (ATRP)[2]to study its oxidative inactivation of enzyme activity, and to screen MIRA ingredients[3]for the proteolysis of isolevuglandins (IsoLGs) proteins[4]
• Biochem/physiol Actions: Aminopeptidase M (APM) is implicated in terminating the actions of certain neuropeptides, like the enkephalins and angiotensin III in the brain. It also metabolizes specific vasoactive peptides. It functions as a receptor for certain viruses, such as coronavirus 229E.[1] APM is a nonspecific peptidase, which rapidly breaks down l-residue peptides.[2]
• Packaging: Please refer to vial label for lot-specific concentration.
• Warning: Toxicity: Harmful (C)
• Unit Definition: One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of Leu-pNA per min at 37°C, pH 7.2.
• Physical form: Crystalline suspension in 3.2 M (NH₄)₂SO₄, 10 mM MgCl₂, 10 mM Tris-HCl, pH 7.5.
• Other Notes: Wachsmuth, E.E., et al. 1966. Biochemistry5, 169.
• Legal Information: CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany