Description

• General description: Native trypsin from bovine pancreas. Serine protease that specifically hydrolyzes amides, peptides and proteins at the C-terminal side of arginine and lysine residues. Inhibitors include aprotinin, DFP, and soybean trypsin inhibitor. Note: 1 MU = 1,000,000 units.
• Warning: Toxicity: Irritant (B)
• Unit Definition: One USP unit is defined as the amount of enzyme that will digest BAEE and cause an increase in absorbance of 0.003 units at 253 nm per min at 25°C, pH 7.6.
• Physical form: Salt-free
• Reconstitution: Following reconstitution in HCl, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Dilute into aqueous buffers (at neutral pH) just prior to use. Following reconstitution in H₂O or buffer, use immediately or aliquot and freeze (-70°C) to minimize autolysis. HCl stock solutions are stable for up to 1 week at 4°C or for up to 1 month at -20°C. H₂O or buffer stock solutions are stable for up to 1 month at -70°C.
• Other Notes: Bergmeyer, H.U. 1983. Methods of Enzymatic Analysis, 3rd ed. Vol. 3, 17.Kostka, V., and Carpenter, F.H. 1964. J. Biol. Chem.239, 1799.Hummel, B.C.W. 1955. Can. J. Biochem. Physiol.37, 1393.
• Legal Information: CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany