C5233
Carboxypeptidase B from human pancreas
50-55 units/mg protein carboxypeptidase B
Manufacturer: Sigma Aldrich
CAS Number: 9025-24-5
Synonym(S): Peptidyl-L-Lysine[L-arginine] hydrolase
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 μG | C5233-100-μG | In Stock | ₹ 1,73,548.50 |
C5233 - 100 μG
In Stock
Quantity
1
Base Price: ₹ 1,73,548.50
GST (18%): ₹ 31,238.73
Total Price: ₹ 2,04,787.23
biological source
human pancreas
Quality Level
200
form
solution
specific activity
50-55 units/mg protein carboxypeptidase B
impurities
≤0.2% chymotrypsin≤0.2% trypsin≤1 unit/mg protein carboxypeptidase A
UniProt accession no.
P15086
shipped in
dry ice
storage temp.
−20°C
InChI
1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)
InChI key
TWURVFFNODFJBJ-UHFFFAOYSA-N
Other Options
| Image | Product Name | Manufacturer | Price Range | |
|---|---|---|---|---|
 | MilliporeSigma™ Calbiochem™ Carboxypeptidase B, Porcine Pancreas | -- | ₹ 13,024.80 | |
| | Sigma Aldrich Fine Chemicals Biosciences Carboxypeptidase B from porcine pancreas lyophilized powder | 9025-24-5 | MFCD00081476 | 5mg | -- | ₹ 33,644.76 | |
| | Sigma Aldrich Fine Chemicals Biosciences Carboxypeptidase B from porcine pancreas lyophilized powder | 9025-24-5 | MFCD00081476 | 1MG | -- | ₹ 9,684.54 | |
| | Carboxypeptidase B from porcine pancreas | -- | ₹ 10,478.40 - ₹ 34,598.70 | |
| | Carboxypeptidase B, Porcine Pancreas | -- | ₹ 11,510.00 | |
| | 9025-24-5 | Carboxypeptidase B | -- | ₹ 10,181.64 - ₹ 25,496.88 |
Related Products
Description
- General description: Carboxypeptidase B is mapped to human chromosome 3q24.[1] Carboxypeptidase B belongs to A/B subfamily of carboxypeptidases.[2]
- Application: Carboxypeptidase B from Sigma has been used as a reference for assaying carboxypeptidase activity in lysed pituitary granules derived from the anterior and intermediate lobes of rat.[3] The enzyme has also been used to digest plasma samples by removing C-terminal basic amino acids, to get a distinct band for each allotype during C4 electrophoresis.[4]
- Biochem/physiol Actions: Carboxypeptidase B (or peptidyl-L-lysine (-L-arginine) hydrolase) catalyzes the hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-terminal position of polypeptides. It has been shown to be a single polypeptide of 34,000 Da. Trypsin is capable of converting native enzyme to the active enzyme, carboxypeptidase B II in vitro.[5] The optimum pH is found to be 9.0. The enzyme may be used for sequence analysis by successive cleavage of C-terminal basic amino acids. It can also be used as a serum marker for the diagnosis of acute pancreatitis.
- Unit Definition: One unit will hydrolyze 1 μmole of hippuryl-L-arginine per minute at pH 7.7 at 25 °C
- Physical form: Solution in 0.05 M NaOAc pH 5.0 + 1.0 M NaCl + 0.01% NaN3
SAFETY INFORMATION
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable