K1684
Thermo Scientific™ GeneBLAzer™ TRα-UAS-blaHEK 293T Assay Kit
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1684-Each-of-1 | In Stock | ₹ 8,98,380.00 |
K1684 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 8,98,380.00
GST (18%): ₹ 1,61,708.40
Total Price: ₹ 10,60,088.40
Alternate Agonists
9-cis-retinoic acid, TTNPB, CD437, AM 580
Assay Entry
Cell-based beta lactamase reporter gene
Content And Storage
TR alpha-UAS-bla HEK 293T cells (K1260)Shipping:Dry IceStorage:-80°C (liquid nitrogen)
Primary Agonist
All-trans-retinoic Acid
Quantity
1 Plate
Target Entry
THRA, TR alpha, NR1A1
Cell Line
HEK 293T
Druggable Target
Nuclear Receptors
Antagonists
AGN 193109
Cell State
Dividing Cells
Detection Method
Fluorescence
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Type
Detection Assay Kit
System Type
GeneBLAzer™
Related Products
Description
- TR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Thyroid hormone receptor alpha(TR alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line
- GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS)
- When an agonist binds to the LBD of the GAL4 (DBD)-TR alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase
- TR alpha-UASbla HEK 293T 293 cells are functionally validated for Z' and EC 50 concentrations of T3 Thryoid hormone
- In addition, TR alpha-UAS-bla HEK 293T 293 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request)
- Additional testing data using alternate stimuli are also available.